Bullpen assistant Strech Suba, a fixture in the Astros dugout and clubhouse since 1978, will not return to the team for the 2013 season.
Suba was told on Monday that his contract will not be renewed.
"The Astros have been great to me," Suba said. "I was given a job after having been released that I had been with for 35 years.
"They always treated me well and I couldn't have asked for any better way to have a career, especially being born and raised in Houston."
Suba spent one season as a catcher with the Astros rookie league team in 1978 in Sarasota, Fla.
"When I was released as a player in the minor leagues back in '78 and then two hours later (former Astros president) Tal Smith gave me the bullpen catchers job."
And Suba kept that job through the 2012 season.
Suba, who lives in San Diego, Calif., said he is open to working for the Astros in another capacity.
"We recognize the many years of service that Strech has provided for the Houston Astros, but we've decided to go in another direction with our on-field staff," said Jeff Luhnow, Astros general manager, in a statement released by the team.
"We thank Strech for all he has done for our organization and wish him the best in the future."
Meanwhile former Astros pitching coach Burt Hooton was not with the club nearly as long as Suba, but he joins him as a former member of the organization.
Hooton confirms that his contract as the Astros pitching coach at Triple-A Oklahoma City was not renewed.
Hooton, who was the pitching coach for the Astros from 2000-2004, had been working in Houston's minor league system for the last eight years.
"I was told they weren't renewing my contract," Hooton said. "They have all the reasons. I don't have any answers.
"13 years with one club, I enjoyed it. It was a good run. No hard feelings. They have a new ownership group and they can do with it what they want, and they didn't want me to be a part of it.
Hooton, who played 15 years in the Major Leagues, is not ready to say his coaching career is over.
"I'm not ready," Hooton said. "I think I have a lot to offer still.
"I enjoy it. I love what I do."